maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10 ** relaxed parameter
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
57721 target sequences.
57721 total sequences.
85714244 total DNA bases.
15735 is the longest sequence length.
4 CPU used in this computation.
15565 sequences were dead due to short overlaps with others.
18094 sequences were dead due to long overlaps with others.
390 sequences were dead with no good probe candidates.
56394 initial best probe candidates detected.
2731 sequences without probe at 63.53 C, 429 below, 2302 above.
2728 sequences without probe at 63.63 C, 460 below, 2268 above.
2647 sequences without probe at 64.24 C, 647 below, 2000 above.
2623 sequences without probe at 64.89 C, 872 below, 1751 above.
2611 sequences without probe at 65.14 C, 955 below, 1656 above.
2648 sequences without probe at 65.69 C, 1226 below, 1422 above.
2694 sequences without probe at 65.88 C, 1342 below, 1352 above.
2814 sequences without probe at 66.24 C, 1564 below, 1250 above.
2611 sequences without probe due to temperature conflicts.
21061 total oligo probes designed for 21061 sequences.
60.14 C is the maximum estimated probe-nontarget temperature.
70.14 C is the minimum estimated probe-target temperature.
65.14 C is the optimal estimated experiment temperature.