maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
57721 target sequences.
57721 total sequences.
85714244 total DNA bases.
15735 is the longest sequence length.
4 CPU used in this computation.
15565 sequences were dead due to short overlaps with others.
18094 sequences were dead due to long overlaps with others.
2789 sequences were dead with no good probe candidates.
47923 initial best probe candidates detected.
8637 sequences without probe at 63.02 C, 3548 below, 5089 above.
8680 sequences without probe at 63.09 C, 3638 below, 5042 above.
8676 sequences without probe at 63.12 C, 3661 below, 5015 above.
8678 sequences without probe at 63.22 C, 3754 below, 4924 above.
8674 sequences without probe at 63.32 C, 3869 below, 4805 above.
8668 sequences without probe at 63.44 C, 3979 below, 4689 above.
8741 sequences without probe at 63.59 C, 4180 below, 4561 above.
8776 sequences without probe at 63.64 C, 4249 below, 4527 above.
8785 sequences without probe at 63.67 C, 4274 below, 4511 above.
8823 sequences without probe at 63.74 C, 4360 below, 4463 above.
8851 sequences without probe at 63.81 C, 4430 below, 4421 above.
8637 sequences without probe due to temperature conflicts.
12636 total oligo probes designed for 12636 sequences.
53.02 C is the maximum estimated probe-nontarget temperature.
73.02 C is the minimum estimated probe-target temperature.
63.02 C is the optimal estimated experiment temperature.