maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
57721 target sequences.
57721 total sequences.
85714244 total DNA bases.
15735 is the longest sequence length.
3859 sequences were dead due to short overlaps with others.
13409 sequences were dead due to long overlaps with others.
1164 sequences were dead with no good probe candidates.
141966 initial best probe candidates detected.
3458 sequences without probe due to temperature conflicts.
35831 total oligo probes designed for 35831 sequences.
66.93 C is the optimal estimated experiment temperature.
total time elapsed 52693 seconds