maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
57721 target sequences.
57721 total sequences.
85714244 total DNA bases.
15735 is the longest sequence length.
3859 sequences were dead due to short overlaps with others.
13409 sequences were dead due to long overlaps with others.
3918 sequences were dead with no good probe candidates.
126986 initial best probe candidates detected.
10989 sequences without probe due to temperature conflicts.
25546 total oligo probes designed for 25546 sequences.
64.39 C is the optimal estimated experiment temperature.
total time elapsed 29180 seconds