maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 17
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0
57721 target sequences.
57721 total sequences.
85714244 total DNA bases.
15735 is the longest sequence length.
1388 sequences were dead due to short overlaps with others.
10986 sequences were dead due to long overlaps with others.
4985 sequences were dead with no good probe candidates.
151620 initial best probe candidates detected.
11747 sequences without probe due to temperature conflicts.
28615 total oligo probes designed for 28615 sequences.
64.44 C is the optimal estimated experiment temperature.
total time elapsed 156507 seconds