Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 17
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Fri 13 May 2005 04:52:20 PM CDT
Step 1 of 6: Constructing the suffix array...
18962 target sequences.
18962 total sequences.
32217720 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
16380 is the longest common overlap region length among sequences.
485.55 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
4 CPU(s) used in this computation; step 3 is slower, be patient....
3 sequences have no unique probe due to short overlaps with others.
6466 sequences have no unique probe due to long overlaps with others.
113 sequences have no good probe candidates due to temperature limit.
55123 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
6530 potentially sharable long common overlap regions identified.
4194 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
4 CPU(s) used in this computation; step 5 is also slow, be patient...
13 host sequences were not useful due to short overlaps.
202 host sequences were taken by the other host sequences.
301 host sequences produce no probes due to temperature limit.
19033 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
1855 sequences/regions without probe at 65.02 C, 542 below, 1313 above.
1750 sequences/regions without probe at 65.35 C, 608 below, 1142 above.
1692 sequences/regions without probe at 65.78 C, 697 below, 995 above.
1637 sequences/regions without probe at 66.23 C, 783 below, 854 above.
1629 sequences/regions without probe at 66.32 C, 807 below, 822 above.
1635 sequences/regions without probe at 66.70 C, 913 below, 722 above.
1616 sequences/regions without probe at 66.43 C, 834 below, 782 above.
1616 sequences/regions without probe at 20.00 separation temperature.
11337 unique oligo probes designed for 11337 sequences.
3277 shared oligo probes designed for 6821 additional sequences.
26 maximum number of sequences sharing the same oligo probe.
2.81 average number of sequences sharing the same oligo probe.
56.42 C is the maximum estimated probe-nontarget temperature.
76.43 C is the minimum estimated probe-target temperature.
66.43 C is the optimal estimated experiment temperature.
14614 total oligo probes designed for 18158 sequences.
Job completed on Fri 13 May 2005 07:11:22 PM CDT
Total time elapsed 02h:19m:02s.