Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Tue 17 May 2005 09:45:22 AM CDT
Step 1 of 6: Constructing the suffix array...
28952 target sequences.
28952 total sequences.
36327482 total DNA bases.
16011 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
10281 is the longest common overlap region length among sequences.
79.57 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
1856 sequences have no unique probe due to short overlaps with others.
5873 sequences have no unique probe due to long overlaps with others.
261 sequences have no good probe candidates due to temperature limit.
69493 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
8946 potentially sharable long common overlap regions identified.
4950 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
713 host sequences were not useful due to short overlaps.
1502 host sequences were taken by the other host sequences.
165 host sequences produce no probes due to temperature limit.
7968 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
953 sequences/regions without probe at 63.21 C, 131 below, 822 above.
931 sequences/regions without probe at 63.46 C, 152 below, 779 above.
911 sequences/regions without probe at 63.69 C, 174 below, 737 above.
904 sequences/regions without probe at 64.38 C, 277 below, 627 above.
943 sequences/regions without probe at 65.01 C, 401 below, 542 above.
981 sequences/regions without probe at 65.28 C, 472 below, 509 above.
1008 sequences/regions without probe at 65.52 C, 523 below, 485 above.
992 sequences/regions without probe at 65.36 C, 488 below, 504 above.
904 sequences/regions without probe at 10.00 separation temperature.
20203 unique oligo probes designed for 20203 sequences.
2278 shared oligo probes designed for 4330 additional sequences.
15 maximum number of sequences sharing the same oligo probe.
2.19 average number of sequences sharing the same oligo probe.
59.37 C is the maximum estimated probe-nontarget temperature.
69.40 C is the minimum estimated probe-target temperature.
64.38 C is the optimal estimated experiment temperature.
22481 total oligo probes designed for 24533 sequences.
Job completed on Tue 17 May 2005 10:30:07 AM CDT
Total time elapsed 00h:44m:45s.