Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Wed 18 May 2005 09:26:13 AM CDT
Step 1 of 6: Constructing the suffix array...
28952 target sequences.
28952 total sequences.
36327482 total DNA bases.
16011 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
10281 is the longest common overlap region length among sequences.
79.57 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
113 sequences have no unique probe due to short overlaps with others.
4639 sequences have no unique probe due to long overlaps with others.
196 sequences have no good probe candidates due to temperature limit.
106522 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
9115 potentially sharable long common overlap regions identified.
4982 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
372 host sequences were not useful due to short overlaps.
901 host sequences were taken by the other host sequences.
538 host sequences produce no probes due to temperature limit.
13548 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
499 sequences/regions without probe at 63.07 C, 90 below, 409 above.
485 sequences/regions without probe at 63.37 C, 108 below, 377 above.
482 sequences/regions without probe at 63.45 C, 112 below, 370 above.
488 sequences/regions without probe at 63.84 C, 139 below, 349 above.
526 sequences/regions without probe at 64.73 C, 241 below, 285 above.
557 sequences/regions without probe at 65.03 C, 291 below, 266 above.
482 sequences/regions without probe at 10.00 separation temperature.
23664 unique oligo probes designed for 23664 sequences.
2511 shared oligo probes designed for 4333 additional sequences.
15 maximum number of sequences sharing the same oligo probe.
2.20 average number of sequences sharing the same oligo probe.
58.45 C is the maximum estimated probe-nontarget temperature.
68.49 C is the minimum estimated probe-target temperature.
63.45 C is the optimal estimated experiment temperature.
26175 total oligo probes designed for 27997 sequences.
Job completed on Wed 18 May 2005 02:15:47 PM CDT
Total time elapsed 04h:49m:34s.