Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Tue 17 May 2005 01:39:15 PM CDT
Step 1 of 6: Constructing the suffix array...
28952 target sequences.
28952 total sequences.
36327482 total DNA bases.
16011 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
10281 is the longest common overlap region length among sequences.
79.57 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
113 sequences have no unique probe due to short overlaps with others.
4639 sequences have no unique probe due to long overlaps with others.
748 sequences have no good probe candidates due to temperature limit.
100733 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
9115 potentially sharable long common overlap regions identified.
4982 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
372 host sequences were not useful due to short overlaps.
901 host sequences were taken by the other host sequences.
692 host sequences produce no probes due to temperature limit.
12596 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
2027 sequences/regions without probe at 62.98 C, 1105 below, 922 above.
2048 sequences/regions without probe at 62.72 C, 1050 below, 998 above.
2042 sequences/regions without probe at 62.57 C, 999 below, 1043 above.
2027 sequences/regions without probe at 20.00 separation temperature.
21907 unique oligo probes designed for 21907 sequences.
2457 shared oligo probes designed for 4445 additional sequences.
16 maximum number of sequences sharing the same oligo probe.
2.20 average number of sequences sharing the same oligo probe.
52.98 C is the maximum estimated probe-nontarget temperature.
72.99 C is the minimum estimated probe-target temperature.
62.98 C is the optimal estimated experiment temperature.
24364 total oligo probes designed for 26352 sequences.
Job completed on Tue 17 May 2005 06:30:46 PM CDT
Total time elapsed 04h:51m:31s.