Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 17
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Tue 17 May 2005 07:51:13 PM CDT
Step 1 of 6: Constructing the suffix array...
28952 target sequences.
28952 total sequences.
36327482 total DNA bases.
16011 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
10281 is the longest common overlap region length among sequences.
79.57 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
31 sequences have no unique probe due to short overlaps with others.
4249 sequences have no unique probe due to long overlaps with others.
199 sequences have no good probe candidates due to temperature limit.
112018 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
9197 potentially sharable long common overlap regions identified.
4999 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
247 host sequences were not useful due to short overlaps.
655 host sequences were taken by the other host sequences.
911 host sequences produce no probes due to temperature limit.
14101 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
459 sequences/regions without probe at 63.15 C, 101 below, 358 above.
453 sequences/regions without probe at 63.45 C, 117 below, 336 above.
454 sequences/regions without probe at 63.74 C, 141 below, 313 above.
466 sequences/regions without probe at 63.99 C, 166 below, 300 above.
483 sequences/regions without probe at 64.71 C, 230 below, 253 above.
507 sequences/regions without probe at 65.01 C, 269 below, 238 above.
453 sequences/regions without probe at 10.00 separation temperature.
24174 unique oligo probes designed for 24174 sequences.
2434 shared oligo probes designed for 4125 additional sequences.
15 maximum number of sequences sharing the same oligo probe.
2.19 average number of sequences sharing the same oligo probe.
58.45 C is the maximum estimated probe-nontarget temperature.
68.51 C is the minimum estimated probe-target temperature.
63.45 C is the optimal estimated experiment temperature.
26608 total oligo probes designed for 28299 sequences.
Job completed on Wed 18 May 2005 04:18:07 AM CDT
Total time elapsed 08h:26m:54s.