Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 17
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Mon 16 May 2005 07:56:17 PM CDT
Step 1 of 6: Constructing the suffix array...
28952 target sequences.
28952 total sequences.
36327482 total DNA bases.
16011 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
10281 is the longest common overlap region length among sequences.
79.57 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
31 sequences have no unique probe due to short overlaps with others.
4249 sequences have no unique probe due to long overlaps with others.
673 sequences have no good probe candidates due to temperature limit.
107200 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
9197 potentially sharable long common overlap regions identified.
4999 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
247 host sequences were not useful due to short overlaps.
655 host sequences were taken by the other host sequences.
1067 host sequences produce no probes due to temperature limit.
13226 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
1600 sequences/regions without probe at 63.08 C, 984 below, 616 above.
1591 sequences/regions without probe at 63.01 C, 957 below, 634 above.
1610 sequences/regions without probe at 62.72 C, 883 below, 727 above.
1613 sequences/regions without probe at 62.58 C, 838 below, 775 above.
1649 sequences/regions without probe at 62.22 C, 746 below, 903 above.
1591 sequences/regions without probe at 20.00 separation temperature.
22824 unique oligo probes designed for 22824 sequences.
2477 shared oligo probes designed for 4352 additional sequences.
16 maximum number of sequences sharing the same oligo probe.
2.20 average number of sequences sharing the same oligo probe.
53.01 C is the maximum estimated probe-nontarget temperature.
73.01 C is the minimum estimated probe-target temperature.
63.01 C is the optimal estimated experiment temperature.
25301 total oligo probes designed for 27176 sequences.
Job completed on Tue 17 May 2005 04:22:45 AM CDT
Total time elapsed 08h:26m:28s.