Picky parameter settings for this computation: maximum_oligo_size = 70 minimum_oligo_size = 50 maximum_match_len = 15 minimum_match_len = 10 maximum_gc_content = 70 minimum_gc_content = 30 candidates_per_gene = 5 probes_per_gene = 1 minimum_similarity = 75 minimum_temp_separation = 20 DNA_concentration = 1 Salt_concentration = 75 single_strand_only = 0 Job started on Wed 25 May 2005 11:38:41 AM CDT Step 1 of 6: Constructing the suffix array... 11324 target sequences. 11324 total sequences. 6022273 total DNA bases. 5426 is the longest sequence length. Step 2 of 6: Computing invert and lcp arrays... 982 is the longest common overlap region length among sequences. 13.96 is the average common overlap region length among sequences. Step 3 of 6: Generating unique probe candidates... 2 CPU(s) used in this computation; step 3 is slower, be patient.... 306 sequences have no unique probe due to short overlaps with others. 310 sequences have no unique probe due to long overlaps with others. 48 sequences have no good probe candidates due to temperature limit. 40655 initial unique probe candidates detected. Step 4 of 6: Identifying long common overlap regions... 888 potentially sharable long common overlap regions identified. 619 sequences hosting long common overlap regions identified. Step 5 of 6: Generating shared probe candidates... 2 CPU(s) used in this computation; step 5 is also slow, be patient... 90 host sequences were not useful due to short overlaps. 54 host sequences were taken by the other host sequences. 128 host sequences produce no probes due to temperature limit. 663 additional sharable probe candidates detected. Step 6 of 6: Determining the optimal oligo set... 703 sequences/regions without probe at 59.81 C, 379 below, 324 above. 673 sequences/regions without probe at 59.24 C, 276 below, 397 above. 673 sequences/regions without probe at 20.00 separation temperature. 10083 unique oligo probes designed for 10083 sequences. 154 shared oligo probes designed for 191 additional sequences. 6 maximum number of sequences sharing the same oligo probe. 2.06 average number of sequences sharing the same oligo probe. 49.22 C is the maximum estimated probe-nontarget temperature. 69.24 C is the minimum estimated probe-target temperature. 59.24 C is the optimal estimated experiment temperature. 10237 total oligo probes designed for 10274 sequences. Job completed on Wed 25 May 2005 11:50:16 AM CDT Total time elapsed 00h:11m:35s.