Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Wed 11 May 2005 02:22:55 PM CDT
Step 1 of 6: Constructing the suffix array...
26236 target sequences.
26236 total sequences.
32759147 total DNA bases.
15717 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
9948 is the longest common overlap region length among sequences.
92.75 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
51 sequences have no unique probe due to short overlaps with others.
8465 sequences have no unique probe due to long overlaps with others.
67 sequences have no good probe candidates due to temperature limit.
78067 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
29441 potentially sharable long common overlap regions identified.
11264 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
96 host sequences were not useful due to short overlaps.
1507 host sequences were taken by the other host sequences.
952 host sequences produce no probes due to temperature limit.
35449 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
2124 sequences/regions without probe at 63.31 C, 258 below, 1866 above.
1877 sequences/regions without probe at 64.72 C, 518 below, 1359 above.
1860 sequences/regions without probe at 64.90 C, 561 below, 1299 above.
2049 sequences/regions without probe at 66.46 C, 1161 below, 888 above.
1861 sequences/regions without probe at 65.50 C, 737 below, 1124 above.
1860 sequences/regions without probe at 10.00 separation temperature.
17179 unique oligo probes designed for 17179 sequences.
5330 shared oligo probes designed for 7935 additional sequences.
20 maximum number of sequences sharing the same oligo probe.
2.53 average number of sequences sharing the same oligo probe.
59.90 C is the maximum estimated probe-nontarget temperature.
69.90 C is the minimum estimated probe-target temperature.
64.90 C is the optimal estimated experiment temperature.
22509 total oligo probes designed for 25114 sequences.
Job completed on Wed 11 May 2005 06:44:11 PM CDT
Total time elapsed 04h:21m:16s.