Picky parameter settings for this computation: maximum_oligo_size = 70 minimum_oligo_size = 50 maximum_match_len = 16 minimum_match_len = 10 maximum_gc_content = 70 minimum_gc_content = 30 candidates_per_gene = 5 probes_per_gene = 1 minimum_similarity = 75 minimum_temp_separation = 10 DNA_concentration = 1 Salt_concentration = 75 single_strand_only = 0 Job started on Mon 09 May 2005 05:13:07 PM CDT Step 1 of 6: Constructing the suffix array... 28205 target sequences. 28205 total sequences. 72748721 total DNA bases. 16380 is the longest sequence length. Step 2 of 6: Computing invert and lcp arrays... 16179 is the longest common overlap region length among sequences. 292.92 is the average common overlap region length among sequences. Step 3 of 6: Generating unique probe candidates... 2 CPU(s) used in this computation; step 3 is slower, be patient.... 86 sequences have no unique probe due to short overlaps with others. 6416 sequences have no unique probe due to long overlaps with others. 223 sequences have no good probe candidates due to temperature limit. 94513 initial unique probe candidates detected. Step 4 of 6: Identifying long common overlap regions... 22409 potentially sharable long common overlap regions identified. 8339 sequences hosting long common overlap regions identified. Step 5 of 6: Generating shared probe candidates... 2 CPU(s) used in this computation; step 5 is also slow, be patient... 253 host sequences were not useful due to short overlaps. 2438 host sequences were taken by the other host sequences. 429 host sequences produce no probes due to temperature limit. 24970 additional sharable probe candidates detected. Step 6 of 6: Determining the optimal oligo set... 2078 sequences/regions without probe at 64.22 C, 275 below, 1803 above. 1974 sequences/regions without probe at 64.75 C, 354 below, 1620 above. 1877 sequences/regions without probe at 65.21 C, 417 below, 1460 above. 1841 sequences/regions without probe at 65.51 C, 470 below, 1371 above. 1813 sequences/regions without probe at 66.07 C, 611 below, 1202 above. 1807 sequences/regions without probe at 66.54 C, 737 below, 1070 above. 1792 sequences/regions without probe at 66.70 C, 772 below, 1020 above. 1808 sequences/regions without probe at 67.07 C, 884 below, 924 above. 1913 sequences/regions without probe at 67.61 C, 1072 below, 841 above. 1845 sequences/regions without probe at 67.25 C, 954 below, 891 above. 1792 sequences/regions without probe at 10.00 separation temperature. 20702 unique oligo probes designed for 20702 sequences. 3325 shared oligo probes designed for 5744 additional sequences. 23 maximum number of sequences sharing the same oligo probe. 2.65 average number of sequences sharing the same oligo probe. 61.70 C is the maximum estimated probe-nontarget temperature. 71.70 C is the minimum estimated probe-target temperature. 66.70 C is the optimal estimated experiment temperature. 24027 total oligo probes designed for 26446 sequences. Job completed on Tue 10 May 2005 01:42:49 AM CDT Total time elapsed 08h:29m:42s.