Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 17
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Mon 09 May 2005 05:18:33 PM CDT
Step 1 of 6: Constructing the suffix array...
28205 target sequences.
28205 total sequences.
72748721 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
16179 is the longest common overlap region length among sequences.
292.92 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
4 CPU(s) used in this computation; step 3 is slower, be patient....
14 sequences have no unique probe due to short overlaps with others.
5338 sequences have no unique probe due to long overlaps with others.
288 sequences have no good probe candidates due to temperature limit.
101819 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
22730 potentially sharable long common overlap regions identified.
8380 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
4 CPU(s) used in this computation; step 5 is also slow, be patient...
151 host sequences were not useful due to short overlaps.
1648 host sequences were taken by the other host sequences.
953 host sequences produce no probes due to temperature limit.
29785 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
2336 sequences/regions without probe at 64.43 C, 303 below, 2033 above.
2286 sequences/regions without probe at 64.68 C, 332 below, 1954 above.
2168 sequences/regions without probe at 65.11 C, 388 below, 1780 above.
2028 sequences/regions without probe at 65.92 C, 527 below, 1501 above.
2022 sequences/regions without probe at 66.12 C, 584 below, 1438 above.
1990 sequences/regions without probe at 66.32 C, 624 below, 1366 above.
1967 sequences/regions without probe at 66.76 C, 729 below, 1238 above.
1979 sequences/regions without probe at 67.19 C, 848 below, 1131 above.
2004 sequences/regions without probe at 67.39 C, 922 below, 1082 above.
2032 sequences/regions without probe at 67.58 C, 996 below, 1036 above.
2089 sequences/regions without probe at 67.91 C, 1135 below, 954 above.
2030 sequences/regions without probe at 67.61 C, 1004 below, 1026 above.
1967 sequences/regions without probe at 10.00 separation temperature.
21802 unique oligo probes designed for 21802 sequences.
3278 shared oligo probes designed for 5275 additional sequences.
22 maximum number of sequences sharing the same oligo probe.
2.65 average number of sequences sharing the same oligo probe.
61.76 C is the maximum estimated probe-nontarget temperature.
71.77 C is the minimum estimated probe-target temperature.
66.76 C is the optimal estimated experiment temperature.
25080 total oligo probes designed for 27077 sequences.
Job completed on Tue 10 May 2005 02:17:07 AM CDT
Total time elapsed 08h:58m:34s.