Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Thu 19 May 2005 09:54:07 AM CDT
Step 1 of 6: Constructing the suffix array...
58579 target sequences.
58579 total sequences.
39022169 total DNA bases.
8251 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
3266 is the longest common overlap region length among sequences.
24.32 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
1631 sequences have no unique probe due to short overlaps with others.
7467 sequences have no unique probe due to long overlaps with others.
4216 sequences have no good probe candidates due to temperature limit.
151126 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
24624 potentially sharable long common overlap regions identified.
13012 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
1718 host sequences were not useful due to short overlaps.
3688 host sequences were taken by the other host sequences.
3693 host sequences produce no probes due to temperature limit.
6928 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
14850 sequences/regions without probe at 63.41 C, 5638 below, 9212 above.
14838 sequences/regions without probe at 63.52 C, 5783 below, 9055 above.
14874 sequences/regions without probe at 63.63 C, 5944 below, 8930 above.
14869 sequences/regions without probe at 63.67 C, 6018 below, 8851 above.
14901 sequences/regions without probe at 63.78 C, 6241 below, 8660 above.
14964 sequences/regions without probe at 63.91 C, 6461 below, 8503 above.
14977 sequences/regions without probe at 63.94 C, 6512 below, 8465 above.
15009 sequences/regions without probe at 63.98 C, 6592 below, 8417 above.
15124 sequences/regions without probe at 64.12 C, 6910 below, 8214 above.
15249 sequences/regions without probe at 64.25 C, 7169 below, 8080 above.
15341 sequences/regions without probe at 64.35 C, 7359 below, 7982 above.
15369 sequences/regions without probe at 64.38 C, 7433 below, 7936 above.
15469 sequences/regions without probe at 64.49 C, 7662 below, 7807 above.
15506 sequences/regions without probe at 64.55 C, 7789 below, 7717 above.
15487 sequences/regions without probe at 64.51 C, 7704 below, 7783 above.
14838 sequences/regions without probe at 20.00 separation temperature.
32888 unique oligo probes designed for 32888 sequences.
1445 shared oligo probes designed for 1875 additional sequences.
5 maximum number of sequences sharing the same oligo probe.
2.11 average number of sequences sharing the same oligo probe.
53.52 C is the maximum estimated probe-nontarget temperature.
73.52 C is the minimum estimated probe-target temperature.
63.52 C is the optimal estimated experiment temperature.
34333 total oligo probes designed for 34763 sequences.
Job completed on Thu 19 May 2005 05:53:00 PM CDT
Total time elapsed 07h:58m:53s.