Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 17
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Thu 19 May 2005 09:52:55 PM CDT
Step 1 of 6: Constructing the suffix array...
58579 target sequences.
58579 total sequences.
39022169 total DNA bases.
8251 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
3266 is the longest common overlap region length among sequences.
24.32 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
734 sequences have no unique probe due to short overlaps with others.
5791 sequences have no unique probe due to long overlaps with others.
4433 sequences have no good probe candidates due to temperature limit.
169184 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
24846 potentially sharable long common overlap regions identified.
13071 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
1037 host sequences were not useful due to short overlaps.
2572 host sequences were taken by the other host sequences.
5924 host sequences produce no probes due to temperature limit.
6134 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
14310 sequences/regions without probe at 63.53 C, 5200 below, 9110 above.
14363 sequences/regions without probe at 63.67 C, 5428 below, 8935 above.
14385 sequences/regions without probe at 63.75 C, 5552 below, 8833 above.
14386 sequences/regions without probe at 63.82 C, 5658 below, 8728 above.
14411 sequences/regions without probe at 63.93 C, 5852 below, 8559 above.
14426 sequences/regions without probe at 63.98 C, 5924 below, 8502 above.
14434 sequences/regions without probe at 64.01 C, 5988 below, 8446 above.
14531 sequences/regions without probe at 64.13 C, 6218 below, 8313 above.
14659 sequences/regions without probe at 64.26 C, 6474 below, 8185 above.
14746 sequences/regions without probe at 64.38 C, 6693 below, 8053 above.
14766 sequences/regions without probe at 64.42 C, 6771 below, 7995 above.
14824 sequences/regions without probe at 64.51 C, 6940 below, 7884 above.
14849 sequences/regions without probe at 64.55 C, 7015 below, 7834 above.
14905 sequences/regions without probe at 64.63 C, 7180 below, 7725 above.
14920 sequences/regions without probe at 64.68 C, 7269 below, 7651 above.
15062 sequences/regions without probe at 64.80 C, 7531 below, 7531 above.
14310 sequences/regions without probe at 20.00 separation temperature.
35664 unique oligo probes designed for 35664 sequences.
1127 shared oligo probes designed for 1444 additional sequences.
5 maximum number of sequences sharing the same oligo probe.
2.09 average number of sequences sharing the same oligo probe.
53.53 C is the maximum estimated probe-nontarget temperature.
73.53 C is the minimum estimated probe-target temperature.
63.53 C is the optimal estimated experiment temperature.
36791 total oligo probes designed for 37108 sequences.
Job completed on Fri 20 May 2005 05:57:18 AM CDT
Total time elapsed 08h:04m:23s.