Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Fri 20 May 2005 07:33:56 AM CDT
Step 1 of 6: Constructing the suffix array...
9518 target sequences.
9518 total sequences.
10749024 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
5295 is the longest common overlap region length among sequences.
35.36 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
2972 sequences have no unique probe due to short overlaps with others.
2275 sequences have no unique probe due to long overlaps with others.
5 sequences have no good probe candidates due to temperature limit.
11238 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
3172 potentially sharable long common overlap regions identified.
1569 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
476 host sequences were not useful due to short overlaps.
728 host sequences were taken by the other host sequences.
55 host sequences produce no probes due to temperature limit.
673 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
75 sequences/regions without probe at 56.72 C, 0 below, 75 above.
48 sequences/regions without probe at 59.84 C, 8 below, 40 above.
96 sequences/regions without probe at 61.32 C, 67 below, 29 above.
48 sequences/regions without probe at 10.00 separation temperature.
4229 unique oligo probes designed for 4229 sequences.
233 shared oligo probes designed for 343 additional sequences.
7 maximum number of sequences sharing the same oligo probe.
2.20 average number of sequences sharing the same oligo probe.
54.80 C is the maximum estimated probe-nontarget temperature.
64.87 C is the minimum estimated probe-target temperature.
59.84 C is the optimal estimated experiment temperature.
4462 total oligo probes designed for 4572 sequences.
Job completed on Fri 20 May 2005 07:38:35 AM CDT
Total time elapsed 00h:04m:39s.