Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 16
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Fri 20 May 2005 08:04:21 AM CDT
Step 1 of 6: Constructing the suffix array...
9518 target sequences.
9518 total sequences.
10749024 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
5295 is the longest common overlap region length among sequences.
35.36 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
2121 sequences have no unique probe due to short overlaps with others.
1952 sequences have no unique probe due to long overlaps with others.
99 sequences have no good probe candidates due to temperature limit.
17315 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
3354 potentially sharable long common overlap regions identified.
1623 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
405 host sequences were not useful due to short overlaps.
646 host sequences were taken by the other host sequences.
121 host sequences produce no probes due to temperature limit.
1153 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
300 sequences/regions without probe at 56.39 C, 118 below, 182 above.
321 sequences/regions without probe at 56.69 C, 162 below, 159 above.
300 sequences/regions without probe at 20.00 separation temperature.
5119 unique oligo probes designed for 5119 sequences.
305 shared oligo probes designed for 450 additional sequences.
41 maximum number of sequences sharing the same oligo probe.
2.31 average number of sequences sharing the same oligo probe.
46.38 C is the maximum estimated probe-nontarget temperature.
66.40 C is the minimum estimated probe-target temperature.
56.39 C is the optimal estimated experiment temperature.
5424 total oligo probes designed for 5569 sequences.
Job completed on Fri 20 May 2005 08:23:33 AM CDT
Total time elapsed 00h:19m:12s.