Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 17
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Fri 20 May 2005 09:42:40 AM CDT
Step 1 of 6: Constructing the suffix array...
9518 target sequences.
9518 total sequences.
10749024 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
5295 is the longest common overlap region length among sequences.
35.36 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
2 CPU(s) used in this computation; step 3 is slower, be patient....
1770 sequences have no unique probe due to short overlaps with others.
1787 sequences have no unique probe due to long overlaps with others.
21 sequences have no good probe candidates due to temperature limit.
20379 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
3481 potentially sharable long common overlap regions identified.
1664 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
2 CPU(s) used in this computation; step 5 is also slow, be patient...
373 host sequences were not useful due to short overlaps.
599 host sequences were taken by the other host sequences.
155 host sequences produce no probes due to temperature limit.
1489 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
108 sequences/regions without probe at 56.45 C, 0 below, 108 above.
65 sequences/regions without probe at 59.24 C, 1 below, 64 above.
136 sequences/regions without probe at 60.83 C, 93 below, 43 above.
65 sequences/regions without probe at 10.00 separation temperature.
5889 unique oligo probes designed for 5889 sequences.
361 shared oligo probes designed for 495 additional sequences.
41 maximum number of sequences sharing the same oligo probe.
2.29 average number of sequences sharing the same oligo probe.
54.07 C is the maximum estimated probe-nontarget temperature.
64.32 C is the minimum estimated probe-target temperature.
59.24 C is the optimal estimated experiment temperature.
6250 total oligo probes designed for 6384 sequences.
Job completed on Fri 20 May 2005 10:26:13 AM CDT
Total time elapsed 00h:43m:33s.