Picky parameter settings for this computation: maximum_oligo_size = 70 minimum_oligo_size = 50 maximum_match_len = 17 minimum_match_len = 10 maximum_gc_content = 70 minimum_gc_content = 10 candidates_per_gene = 5 probes_per_gene = 1 minimum_similarity = 75 minimum_temp_separation = 10 DNA_concentration = 1 Salt_concentration = 75 single_strand_only = 0 Job started on Fri 20 May 2005 12:06:21 PM CDT Step 1 of 6: Constructing the suffix array... 9518 target sequences. 9518 total sequences. 10749024 total DNA bases. 16380 is the longest sequence length. Step 2 of 6: Computing invert and lcp arrays... 5295 is the longest common overlap region length among sequences. 35.36 is the average common overlap region length among sequences. Step 3 of 6: Generating unique probe candidates... 4 CPU(s) used in this computation; step 3 is slower, be patient.... 732 sequences have no unique probe due to short overlaps with others. 1501 sequences have no unique probe due to long overlaps with others. 30 sequences have no good probe candidates due to temperature limit. 26465 initial unique probe candidates detected. Step 4 of 6: Identifying long common overlap regions... 3481 potentially sharable long common overlap regions identified. 1664 sequences hosting long common overlap regions identified. Step 5 of 6: Generating shared probe candidates... 4 CPU(s) used in this computation; step 5 is also slow, be patient... 280 host sequences were not useful due to short overlaps. 535 host sequences were taken by the other host sequences. 204 host sequences produce no probes due to temperature limit. 2096 additional sharable probe candidates detected. Step 6 of 6: Determining the optimal oligo set... 195 sequences/regions without probe at 55.31 C, 75 below, 120 above. 316 sequences/regions without probe at 57.02 C, 231 below, 85 above. 195 sequences/regions without probe at 10.00 separation temperature. 7096 unique oligo probes designed for 7096 sequences. 385 shared oligo probes designed for 527 additional sequences. 41 maximum number of sequences sharing the same oligo probe. 2.34 average number of sequences sharing the same oligo probe. 50.28 C is the maximum estimated probe-nontarget temperature. 60.32 C is the minimum estimated probe-target temperature. 55.31 C is the optimal estimated experiment temperature. 7481 total oligo probes designed for 7623 sequences. Job completed on Fri 20 May 2005 01:01:53 PM CDT Total time elapsed 00h:55m:32s.