Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 17
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 15
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Sun 01 May 2005 07:28:09 PM CDT
Step 1 of 6: Constructing the suffix array...
61251 target sequences.
61251 total sequences.
94194626 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
9306 is the longest common overlap region length among sequences.
99.94 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
4 CPU(s) used in this computation; step 3 is slower, be patient....
805 sequences have no unique probe due to short overlaps with others.
13674 sequences have no unique probe due to long overlaps with others.
3570 sequences have no good probe candidates due to temperature limit.
167300 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
54598 potentially sharable long common overlap regions identified.
19869 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
4 CPU(s) used in this computation; step 5 is also slow, be patient...
1235 host sequences were not useful due to short overlaps.
10447 host sequences were taken by the other host sequences.
2234 host sequences produce no probes due to temperature limit.
25314 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
9822 sequences/regions without probe at 64.43 C, 1987 below, 7835 above.
9775 sequences/regions without probe at 64.48 C, 2004 below, 7771 above.
9683 sequences/regions without probe at 64.71 C, 2184 below, 7499 above.
9593 sequences/regions without probe at 64.91 C, 2336 below, 7257 above.
9505 sequences/regions without probe at 65.16 C, 2549 below, 6956 above.
9480 sequences/regions without probe at 65.37 C, 2754 below, 6726 above.
9487 sequences/regions without probe at 65.45 C, 2829 below, 6658 above.
9468 sequences/regions without probe at 65.65 C, 3032 below, 6436 above.
9475 sequences/regions without probe at 65.75 C, 3146 below, 6329 above.
9478 sequences/regions without probe at 65.91 C, 3314 below, 6164 above.
9492 sequences/regions without probe at 65.97 C, 3381 below, 6111 above.
9508 sequences/regions without probe at 66.05 C, 3460 below, 6048 above.
9526 sequences/regions without probe at 66.14 C, 3562 below, 5964 above.
9525 sequences/regions without probe at 66.21 C, 3647 below, 5878 above.
9618 sequences/regions without probe at 66.39 C, 3900 below, 5718 above.
9647 sequences/regions without probe at 66.55 C, 4113 below, 5534 above.
9687 sequences/regions without probe at 66.66 C, 4262 below, 5425 above.
9686 sequences/regions without probe at 66.66 C, 4271 below, 5415 above.
9741 sequences/regions without probe at 66.77 C, 4408 below, 5333 above.
9772 sequences/regions without probe at 66.88 C, 4529 below, 5243 above.
9833 sequences/regions without probe at 67.01 C, 4697 below, 5136 above.
9851 sequences/regions without probe at 67.07 C, 4780 below, 5071 above.
9954 sequences/regions without probe at 67.25 C, 5035 below, 4919 above.
9468 sequences/regions without probe at 15.00 separation temperature.
35946 unique oligo probes designed for 35946 sequences.
3155 shared oligo probes designed for 4944 additional sequences.
13 maximum number of sequences sharing the same oligo probe.
2.36 average number of sequences sharing the same oligo probe.
58.15 C is the maximum estimated probe-nontarget temperature.
73.15 C is the minimum estimated probe-target temperature.
65.65 C is the optimal estimated experiment temperature.
39101 total oligo probes designed for 40890 sequences.
Job completed on Mon 02 May 2005 06:51:39 AM CDT
Total time elapsed 11h:23m:30s.