Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 10
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Thu 12 May 2005 03:40:38 PM CDT
Step 1 of 6: Constructing the suffix array...
12238 target sequences.
12238 total sequences.
23015888 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
5749 is the longest common overlap region length among sequences.
98.21 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
4 CPU(s) used in this computation; step 3 is slower, be patient....
18 sequences have no unique probe due to short overlaps with others.
4309 sequences have no unique probe due to long overlaps with others.
46 sequences have no good probe candidates due to temperature limit.
34771 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
21913 potentially sharable long common overlap regions identified.
5540 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
4 CPU(s) used in this computation; step 5 is also slow, be patient...
68 host sequences were not useful due to short overlaps.
852 host sequences were taken by the other host sequences.
214 host sequences produce no probes due to temperature limit.
18493 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
740 sequences/regions without probe at 63.28 C, 188 below, 552 above.
737 sequences/regions without probe at 63.97 C, 300 below, 437 above.
791 sequences/regions without probe at 64.55 C, 419 below, 372 above.
737 sequences/regions without probe at 10.00 separation temperature.
7750 unique oligo probes designed for 7750 sequences.
2527 shared oligo probes designed for 4274 additional sequences.
55 maximum number of sequences sharing the same oligo probe.
2.47 average number of sequences sharing the same oligo probe.
58.96 C is the maximum estimated probe-nontarget temperature.
68.98 C is the minimum estimated probe-target temperature.
63.97 C is the optimal estimated experiment temperature.
10277 total oligo probes designed for 12024 sequences.
Job completed on Thu 12 May 2005 04:03:07 PM CDT
Total time elapsed 00h:22m:29s.