Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 15
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Thu 12 May 2005 03:10:21 PM CDT
Step 1 of 6: Constructing the suffix array...
12238 target sequences.
12238 total sequences.
23015888 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
5749 is the longest common overlap region length among sequences.
98.21 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
4 CPU(s) used in this computation; step 3 is slower, be patient....
18 sequences have no unique probe due to short overlaps with others.
4309 sequences have no unique probe due to long overlaps with others.
131 sequences have no good probe candidates due to temperature limit.
34204 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
21913 potentially sharable long common overlap regions identified.
5540 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
4 CPU(s) used in this computation; step 5 is also slow, be patient...
68 host sequences were not useful due to short overlaps.
852 host sequences were taken by the other host sequences.
310 host sequences produce no probes due to temperature limit.
17242 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
3512 sequences/regions without probe at 63.16 C, 1578 below, 1934 above.
3514 sequences/regions without probe at 63.41 C, 1693 below, 1821 above.
3521 sequences/regions without probe at 63.59 C, 1801 below, 1720 above.
3512 sequences/regions without probe at 20.00 separation temperature.
7135 unique oligo probes designed for 7135 sequences.
2400 shared oligo probes designed for 4380 additional sequences.
55 maximum number of sequences sharing the same oligo probe.
2.46 average number of sequences sharing the same oligo probe.
53.16 C is the maximum estimated probe-nontarget temperature.
73.17 C is the minimum estimated probe-target temperature.
63.16 C is the optimal estimated experiment temperature.
9535 total oligo probes designed for 11515 sequences.
Job completed on Thu 12 May 2005 03:32:47 PM CDT
Total time elapsed 00h:22m:26s.