Picky parameter settings for this computation:
maximum_oligo_size = 70
minimum_oligo_size = 50
maximum_match_len = 17
minimum_match_len = 10
maximum_gc_content = 70
minimum_gc_content = 30
candidates_per_gene = 5
probes_per_gene = 1
minimum_similarity = 75
minimum_temp_separation = 20
DNA_concentration = 1
Salt_concentration = 75
single_strand_only = 0

Job started on Fri 13 May 2005 09:30:31 AM CDT
Step 1 of 6: Constructing the suffix array...
12238 target sequences.
12238 total sequences.
23015888 total DNA bases.
16380 is the longest sequence length.
Step 2 of 6: Computing invert and lcp arrays...
5749 is the longest common overlap region length among sequences.
98.21 is the average common overlap region length among sequences.
Step 3 of 6: Generating unique probe candidates...
4 CPU(s) used in this computation; step 3 is slower, be patient....
4 sequences have no unique probe due to short overlaps with others.
3441 sequences have no unique probe due to long overlaps with others.
430 sequences have no good probe candidates due to temperature limit.
37531 initial unique probe candidates detected.
Step 4 of 6: Identifying long common overlap regions...
22128 potentially sharable long common overlap regions identified.
5558 sequences hosting long common overlap regions identified.
Step 5 of 6: Generating shared probe candidates...
4 CPU(s) used in this computation; step 5 is also slow, be patient...
26 host sequences were not useful due to short overlaps.
335 host sequences were taken by the other host sequences.
1021 host sequences produce no probes due to temperature limit.
19010 additional sharable probe candidates detected.
Step 6 of 6: Determining the optimal oligo set...
2973 sequences/regions without probe at 63.12 C, 1420 below, 1553 above.
2988 sequences/regions without probe at 63.48 C, 1580 below, 1408 above.
2981 sequences/regions without probe at 63.22 C, 1461 below, 1520 above.
2973 sequences/regions without probe at 20.00 separation temperature.
7770 unique oligo probes designed for 7770 sequences.
2294 shared oligo probes designed for 3936 additional sequences.
52 maximum number of sequences sharing the same oligo probe.
2.45 average number of sequences sharing the same oligo probe.
53.12 C is the maximum estimated probe-nontarget temperature.
73.12 C is the minimum estimated probe-target temperature.
63.12 C is the optimal estimated experiment temperature.
10064 total oligo probes designed for 11706 sequences.
Job completed on Fri 13 May 2005 11:02:15 AM CDT
Total time elapsed 01h:31m:44s.